3D Clinorotation Affects Drug Sensitivity of Human Ovarian Cancer Cells

Abstract Numerous studies have reported that gravity alteration displays a remarkable influence on the biological processes of cancer cells. Therefore, gravity-related experiments become promising method improving knowledge in field biology and may be useful to detect implications for future treatment. Taking this concept further, we used 3D clinostat (3D-C; 10 rpm changing direction) analyse effect short-term exposure simulated microgravity ( s?g ) cisplatin sensitivity drug resistant human ovarian cells SKOV-3. This allowed us investigate whether altered affects susceptibility Our revealed SKOV-3 morphology efficiency. We observed cell shape, presence membrane blebbing lamellipodia as well lack filopodia when had been cultured 3D-C 2 h. Cytotoxicity, death cycle assays showed an increased percentage apoptotic G0/G1 arrest after with comparison static control, non clinorotated Cell proliferation migration were well. suggest conditions affected cellular mechanisms involved resistance, resulting higher chemotherapeutic. The investigation clarification these results crucial step toward our understanding relationship between resistance chemotherapy response gravitational conditions.